epitope tag

epitope tag
Short peptide sequence that constitutes an epitope for an existing antibody. Widely used in molecular biology to ‘tag’ transgenic proteins (as a translational fusion product) to follow their expression and fate by immunocytochemistry or Western blotting, but without having to raise antibodies against the specific protein. Example: myc tag. See also flag tagging.

Dictionary of molecular biology. 2004.

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  • myc tag — Epitope tag frequently expressed as a translational fusion with a transgenic protein of interest. As there are good antibodies to the myc epitope, this allows localization of the fusion gene product by immunocytochemistry or Western blot, or its… …   Dictionary of molecular biology

  • c-myc tag — Epitope tag (EQKLISEEDL) derived from the c myc protein …   Dictionary of molecular biology

  • VSV-G tag — Epitope tag (YTDIEMNRLGK) derived from the vesicular stomatitis virus G protein …   Dictionary of molecular biology

  • HA tag — (= haemagglutinin tag; hemagglutinin tag (USA) ) Epitope tag (YPYDVPDYA) derived from the haemagglutinin molecule …   Dictionary of molecular biology

  • his tag — (= histidine tag) Epitope tag based on a short stretch (ca 6) of histidine residues. In addition to detection with anti polyhistidine antibodies, such tags permit easy protein purification on nickel based affinity columns …   Dictionary of molecular biology

  • Protein tag — Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes …   Wikipedia

  • Myc-tag — A myc tag is a polypeptide protein tag derived from the c myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from… …   Wikipedia

  • FLAG-tag — FLAG tag, or FLAG octapeptide, is a polypeptide protein tag that is added to a recombinant expressed protein. It is made by inserting a vector into the DNA sequence coding for that specific protein. It can be used for affinity chromatography,… …   Wikipedia

  • flag tagging — Molecular biology technique, in which the gene encoding a protein of interest is mutagenised to include an epitope for which there is a good antibody. The fate of the protein in a transfected cell or transgenic organism can then be followed… …   Dictionary of molecular biology

  • Immunolabeling — is a means of localizing particular antigens within tissue. Immunolabeling is a methodological process where: 1) antibodies are used to identify antigens within an organism and 2) a tag (e.g. through fluorescence, gold beads, epitope tag, etc.)… …   Wikipedia

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